Exploring Rapid and Efficient Protocol for Isolation of Fungal DNA
نویسندگان
چکیده
منابع مشابه
A REPORT OF RAPID AND EFFICIENT METHOD FOR MITOCHONDRIAL DNA
Based on the similarity of prokaryotic DNA to that of mitochondrial DNA, a rapid and efficient method for DNA purification from mitochondrial extracts obtained from different sources, was carried out based on the modification method described by Marmur (1983) which resulted in a high degree of purity (A260/A280=2). In addition we have applied the same technique for the first time to purify...
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DNA isolation is a crucial step of conducting genetic studies in any organism. However, this process is quite difficult when studying fungi because of the need to damage the fungal cell walls of specific structures. In this study, we developed a method for the rapid and efficient isolation of fungal DNA based on simultaneous mechanical and enzymatic cell wall degradation. There are several typi...
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High-quality DNA extraction plays an important role to make sharp bands in the gel electrophoresis and also produces clean chromatograms. Usually, DNA extract is delivered using the modified CTAB method but this method cannot obtain high-quality DNA for molecular analysis from old dried leaves of Bellevalia due to having different chemical compounds which inhibit to obtain a clear DNA extractio...
متن کاملa report of rapid and efficient method for mitochondrial dna
based on the similarity of prokaryotic dna to that of mitochondrial dna, a rapid and efficient method for dna purification from mitochondrial extracts obtained from different sources, was carried out based on the modification method described by marmur (1983) which resulted in a high degree of purity (a260/a280=2). in addition we have applied the same technique for the first time to purify the ...
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ژورنال
عنوان ژورنال: International Journal of Current Microbiology and Applied Sciences
سال: 2017
ISSN: 2319-7692,2319-7706
DOI: 10.20546/ijcmas.2017.603.113